RNeasy Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).	Include DNAse treatment for 15-20min. Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. Use water to elute the RNA that is warmed to ~60`C

Protocol tips
To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
Include DNAse treatment for 15-20min. Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. Use water to elute the RNA that is warmed to ~60`C

Publication protocol

The cells were then grown at 30°C at 250 rpm for 6 h, 5 × 107 cells were harvested and washed, and RNA was extracted with a Qiagen RNeasy minikit (Qiagen, Hilden, Germany). For the analysis of glucose repression, myristic acid and glucose were added at a final concentration of 1% each.

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

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