MagNA Pure LC RNA Isolation Kit

RNA isolation / purification Bacteria - Gram negative Bordetella pertussis

Experiment
RNA isolation / purification Bacteria - Gram negative Bordetella pertussis
Product
MagNA Pure LC RNA Isolation Kit from Roche Lifesciences
Manufacturer
Roche Lifesciences

Protocol tips

Upstream tips
"The MagNA Pure LC 2.0 Instrument, together with its software is required for analysing the yields of this kit.
Do not use more sample than this kit is designed to handle.
Do not allow Wash Buffer I or the Lysis/Binding Buffer to mix with sodium hypochlorite (bleach) solution. This mixture can produce a highly toxic gas"

Publication protocol

RNA isolation from lung tissue with additional determination of RNA concentrations and integrity was performed as described previously.12 RNA from cells in the dLNs was isolated using a MagNA Pure LC RNA Isolation High Performance kit and MagNA Pure System (Roche, Basel, Switzerland). For lung tissue, RNA concentrates from naive and PM mice were analyzed as individual samples, whereas those of SC mice were pooled

Full paper   Login or join for free to view the full paper.

Reviews

MagNA Pure LC RNA Isolation Kit from Roche Lifesciences has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

2 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram negative Bordetella pertussis using MagNA Pure LC RNA Isolation Kit from Roche Lifesciences.

Paper title
Molecular and cellular signatures underlying superior immunity against Bordetella pertussis upon pulmonary vaccination.
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Roche Lifesciences for MagNA Pure LC RNA Isolation Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Bacteria - Gram negative Bordetella pertussis using MagNA Pure LC RNA Isolation Kit from Roche Lifesciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms