High Pure RNA Isolation Kit

RNA isolation / purification Bacteria - Gram negative Chlamydia pneumoniae

Experiment
RNA isolation / purification Bacteria - Gram negative Chlamydia pneumoniae
Product
High Pure RNA Isolation Kit from Roche Lifesciences
Manufacturer
Roche Lifesciences

Protocol tips

Upstream tips
Be careful to create an RNase-free working environment
Protocol tips
Always mix the sample tube well after addition of each reagent.

Publication protocol

Total RNA was extracted from chlamydia-infected or uninfected HEp-2 cells using High Pure RNA Isolation Kit (Roche) according to the manufacturer’s protocol.

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Reviews

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Discussion

Discussion

2 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram negative Chlamydia pneumoniae using High Pure RNA Isolation Kit from Roche Lifesciences.

Paper title
effector chlamydial outer protein N sequesters fructose bisphosphate aldolase A, providing a benefit to bacterial growth
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Manufacturer protocol

Download the product protocol from Roche Lifesciences for High Pure RNA Isolation Kit below.

Download PDF Download manufacturer protocol

Videos

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