SV Total RNA Isolation System

RNA isolation / purification Bacteria - Gram negative Klebsiella pneumoniae

Experiment
RNA isolation / purification Bacteria - Gram negative Klebsiella pneumoniae
Product
SV Total RNA Isolation System from Promega
Manufacturer
Promega

Protocol tips

Protocol tips
Do not freeze-thaw the DNase more than three times after rehydration.
After adding ethanol to the RNA wash solution, label the bottle so that you fellow researchers don't add any extra.
Downstream tips
For better lysis, pipet the RNA Lysis Buffer over the bottom of the well.

Publication protocol

RNA extractions were performed using the SV total RNA isolation kit (Promega), and the corresponding cDNAs were obtained using the Improm-II reverse transcriptase kit (Promega).

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Reviews

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Discussion

Discussion

2 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram negative Klebsiella pneumoniae using SV Total RNA Isolation System from Promega.

Paper title
Evolution to Colistin Resistance by PmrB Sensor Kinase Mutation in KPC-Producing Is Associated with Low-Dosage Colistin Treatment
Full paper
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Manufacturer protocol

Download the product protocol from Promega for SV Total RNA Isolation System below.

Download PDF Download manufacturer protocol

Videos

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