TRI Reagent™ Solution
RNA isolation / purification Bacteria - Gram negative Salmonella typhi
Publication protocol
Inner and outer foreskin pieces and colon biopsies were preserved in RNAlater (Life Technologies) and stored at −80°C. RNA isolations were carried out by placing the thawed tissue in 1ml of TRI Reagent (Life Technologies) and homogenizing it with a homogenizer with wide probe (Omni). Nucleic acid separation used 1-bromo-3-chloropropane (Sigma-Aldrich) and Ribopure Kit (Life Technologies) columns. Blood was collected in Tempus tubes, with RNA isolated according to the Tempus Spin RNA Isolation Kit (Life Technologies).Full paper Login or join for free to view the full paper.
Reviews
TRI Reagent™ Solution from Thermo Fisher Scientific has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
Discussion
Discussion
4 years ago
Author:
Paul G. Macon
Some help with RNA isolation using Trizol
Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?
Papers
Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram negative Salmonella typhi using TRI Reagent™ Solution from Thermo Fisher Scientific.
Manufacturer protocol
Download the product protocol from Thermo Fisher Scientific for TRI Reagent™ Solution below.
Download manufacturer protocol
Videos
Check out videos that might be relevant for performing RNA isolation / purification Bacteria - Gram negative Salmonella typhi using TRI Reagent™ Solution from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.
Outsource your experiment
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment