ReliaPrep™ RNA Miniprep Systems

RNA isolation / purification Bacteria - Gram positive Clostridum botulinum

Experiment

RNA isolation / purification Bacteria - Gram positive Clostridum botulinum

Product

ReliaPrep™ RNA Miniprep Systems from Promega

Manufacturer

Promega

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Before beginning the ReliaPrep™ RNA Cell Miniprep System protocol, four solutions must be prepared.

Upstream tips
Before beginning the ReliaPrep™ RNA Cell Miniprep System protocol, four solutions must be prepared.

Publication protocol

Total RNA was extracted using the ReliaPrep RNA Cell Miniprep System (Promega, Madison, WI) according to the manufacturer’s instructions.

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Reviews

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Discussion

4 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram positive Clostridum botulinum using ReliaPrep™ RNA Miniprep Systems from Promega.

Paper title

CRISPR/Cas9-Mediated Genomic Deletion of the , Gene in Murine P19 Embryonal Carcinoma Cells Results in Low Sensitivity to Botulinum Neurotoxin Type C

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Manufacturer protocol

Download the product protocol from Promega for ReliaPrep™ RNA Miniprep Systems below.

Download manufacturer protocol

Videos

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