InviMag Universal RNA Mini Kit

RNA isolation / purification Bacteria - Gram positive Lactobacillus amylovorus

Experiment

RNA isolation / purification Bacteria - Gram positive Lactobacillus amylovorus

Product

InviMag Universal RNA Mini Kit from Stratec Molecular

Manufacturer

Stratec Molecular

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Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Total RNA was extracted from the lysates by an InviMag Universal RNA Kit (Stratec) using the KingFisher Flex (Thermo Scientific, Langenselbold, Germany) according to the manufacturer’s instructions. The yield of total RNA was estimated spectrophotometrically at 260 nm and isolated RNA was stored at -80°C. RNA quality and integrity was verified using the Agilent 2100 Bioanalyzer (RNA 6000 Nano Chip, Agilent, Waldbronn, Germany).

Protocol tips
Total RNA was extracted from the lysates by an InviMag Universal RNA Kit (Stratec) using the KingFisher Flex (Thermo Scientific, Langenselbold, Germany) according to the manufacturer’s instructions. The yield of total RNA was estimated spectrophotometrically at 260 nm and isolated RNA was stored at -80°C. RNA quality and integrity was verified using the Agilent 2100 Bioanalyzer (RNA 6000 Nano Chip, Agilent, Waldbronn, Germany).

Protocol tips

Total RNA was extracted from the lysates by an InviMag Universal RNA Kit (Stratec) using the KingFisher Flex (Thermo Scientific, Langenselbold, Germany) according to the manufacturer’s instructions. The yield of total RNA was estimated spectrophotometrically at 260 nm and isolated RNA was stored at -80°C. RNA quality and integrity was verified using the Agilent 2100 Bioanalyzer (RNA 6000 Nano Chip, Agilent, Waldbronn, Germany).

Publication protocol

Reviews

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Discussion

4 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram positive Lactobacillus amylovorus using InviMag Universal RNA Mini Kit from Stratec Molecular.

Paper title

Detection and Characterisation of spp. in the Bovine Uterus and Their Influence on Bovine Endometrial Epithelial Cells

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Manufacturer protocol

Download the product protocol from Stratec Molecular for InviMag Universal RNA Mini Kit below.

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Videos

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