AllPrep DNA/RNA/miRNA Universal Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- This kit purifies RNA from a maximum amount of 30 mg fresh or frozen tissue or 20 mg of tissue that has been stabilized with RNAlater RNA Stabilization Reagent or Allprotect Tissue Reagent can generally be processed.	- Perform all centrifugation steps at 20–25ºC in astandard microcentrifuge	- For low A260/A280 value use 10 mM Tris·Cl,* pH 7.5, not RNase-free water, to dilute the sample before measuring purity.

Upstream tips
- This kit purifies RNA from a maximum amount of 30 mg fresh or frozen tissue or 20 mg of tissue that has been stabilized with RNAlater RNA Stabilization Reagent or Allprotect Tissue Reagent can generally be processed.
Protocol tips
- Perform all centrifugation steps at 20–25ºC in astandard microcentrifuge
Downstream tips
- For low A260/A280 value use 10 mM Tris·Cl,* pH 7.5, not RNase-free water, to dilute the sample before measuring purity.

Publication protocol

Total RNA was prepared from isolated whole gonadal adipose tissues using the AllPrep DNA/RNA mini kit (Qiagen, Hilden, Germany).

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Manufacturer protocol

Download the product protocol from Qiagen for AllPrep DNA/RNA/miRNA Universal Kit below.

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