TRI Reagent® MRC

RNA isolation / purification Tissue - Mouse Artery / aorta

Experiment
RNA isolation / purification Tissue - Mouse Artery / aorta
Product
TRI Reagent® MRC from Molecular Research Center, Inc.
Manufacturer
Molecular Research Center, Inc.

Protocol tips

Protocol tips
- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA

- The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Publication protocol

Total RNA was extracted using the TRI Reagent® (Molecular Research Center; Part #: TR118) method, and isolated RNA was quantified using chip-based capillary electrophoresis (Agilent 2100 Bioanalyzer Pico Chip). RNA was digested with RNase free DNase I (Epicentre; Part # D9905K) and purified through minElute columns (Qiagen; Part #: 74204). Final RNA samples were quantified by O.D. measurement and re-quantified using chip-based capillary electrophoresis.

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Discussion

Discussion

1 year ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

2 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

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Manufacturer protocol

Download the product protocol from Molecular Research Center, Inc. for TRI Reagent® MRC below.

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