miRNeasy Mini kit

RNA isolation / purification Tissue - Mouse Bone

RNA isolation / purification Tissue - Mouse Bone
miRNeasy Mini kit from Qiagen

Protocol tips

Protocol tips
- To capture a higher amount of mRNA, modify the amount of lysis buffer :ethanol (from 1:1 to 1:1.5) during the binding step.

Publication protocol

Each tissue was ground (mortar and pestle, under continuous liquid N2 chilling) into a fine powder before RNA extraction. All the tissues were histologically confirmed to be normal, and stored at −80 °C. Total RNA was extracted from tissue by using the miRNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol, including treatment with DNase. RNAs longer than 18 nucleotides were recovered with this method. RNA quality was evaluated with an Agilent Bioanalyzer (Agilent Technologies, Supplementary Table S1). All the samples had RNA integrity numbers (RINs) greater than 7, except for two bone marrow samples (RIN: 5.3 and 6.2) and one vesicular gland sample (RIN: 4.9). In addition, the RINs were not available of one small intestine sample and one large intestine sample. Excluding all the four bone marrow samples and the two samples of unknown RINs, the average RIN was 8.8 for the remaining 66 RNA samples.

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3 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?


4 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Manufacturer protocol

Download the product protocol from Qiagen for miRNeasy Mini kit below.

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