RNeasy Lipid Tissue Mini Kit

RNA isolation / purification Tissue - Mouse Kidney

Experiment
RNA isolation / purification Tissue - Mouse Kidney
Product
RNeasy Lipid Tissue Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- If the RNA yield is low, repeat RNA elution, but incubate the RNeasy Mini spin column on the benchtop for 10 min with RNase-free water before centrifuging.

- All centrifugation steps should be performed at 15–25°C except for phase separation

Publication protocol

Total RNA (tRNA) was extracted from approximately 100 mg of adipose tissue, using the RNeasy lipid kit (Qiagen Benelux, Venlo, Netherlands), with on-column DNAse treatment using RNAse-free DNAse (Qiagen Benelux, Venlo, Netherlands), according to the manufacturer’s protocol. tRNA was measured on a Nanodrop 1000 (Thermo Fisher Scientific, Waltham, MA, USA) and diluted to equal concentrations.

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

4 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Lipid Tissue Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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