|- For lysis/cell disruption check the protocol beforehand to see which reagents/lab materials are needed
|- Minimal DNA is left over using this kit, however if DNA is undesired an additional RNase free DNase treatment is warranted.
Total RNA Purification Kit from Norgen Biotek has not yet been reviewed for this experiment
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2 years ago
Author: Paul G. Macon
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
3 years ago
Author: Aaron Stege
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
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