RNeasy Plus Mini Kit

RNA isolation / purification Tissue - Mouse Oviduct

Experiment
RNA isolation / purification Tissue - Mouse Oviduct
Product
RNeasy Plus Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

The oviducts were separated into three parts, the infundibulum, the ampulla and the isthmus. Total RNA was extracted from each part using an RNeasy Mini Kit (Qiagen), and then cDNA was synthesized using a SuperScript II First-Strand Synthesis System (Invitrogen). To check the expression of PCP genes in the oviduct, PCR analyses were performed using Platinum Taq DNA Polymerase (Invitrogen). The amplification conditions were as follows: denaturation at 94°C for 5 min, 35 cycles of 95°C denaturation for 20 s, 61°C annealing for 30 s and 72°C extension for 30 s. The primer pairs and the product size for each PCP gene are shown in supplementary material Table S1.



Full paper   Login or join for free to view the full paper.

Reviews

RNeasy Plus Mini Kit from Qiagen has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

1 year ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

2 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Oviduct using RNeasy Plus Mini Kit from Qiagen.

Paper title
Celsr1 is required for the generation of polarity at multiple levels of the mouse oviduct.
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Mini Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Tissue - Mouse Oviduct using RNeasy Plus Mini Kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms