|For lysis/cell disruption check the protocol beforehand to see which reagents/lab materials are needed|
|Minimal DNA is left over using this kit, however if DNA is undesired an additional RNase free DNase treatment is warranted.|
Total RNA Purification Kit from Norgen Biotek has not yet been reviewed for this experiment
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2 years ago
Author: Paul G. Macon
Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?
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