Total RNA Purification Kit

RNA isolation / purification Bacteria - Gram negative Haemophilus influenzae

Experiment
RNA isolation / purification Bacteria - Gram negative Haemophilus influenzae
Product
Total RNA Purification Kit from Norgen Biotek
Manufacturer
Norgen Biotek

Protocol tips

Upstream tips
For lysis/cell disruption check the protocol beforehand to see which reagents/lab materials are needed
Downstream tips
Minimal DNA is left over using this kit, however if DNA is undesired an additional RNase free DNase treatment is warranted.

Publication protocol


Microbial cells were grown in 5 ml of TSB with and without solithromycin at the indicated concentrations. Total RNA was isolated from S. aureus and S. pneumoniae by phenol and CHCl3 extraction (15). RNA was isolated from H. influenzae cells using the total RNA isolation kit from Norgen (Thorold, Canada). RNA quality was examined using an Agilent Bioanalyzer 2100 and the RNA 6000 lab on a chip; 0.5 to 1 μg of total RNA was loaded onto each well of the RNA 6000 chip. Sample preparation and analysis were carried out according to manufacturer's recommendations for total RNA analysis.

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Reviews

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Discussion

Discussion

2 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram negative Haemophilus influenzae using Total RNA Purification Kit from Norgen Biotek.

Paper title
Solithromycin Inhibition of Protein Synthesis and Ribosome Biogenesis in , , and
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Manufacturer protocol

Download the product protocol from Norgen Biotek for Total RNA Purification Kit below.

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