Flotillin-1 siRNA (h)

siRNA / miRNA gene silencing Human - A2780 FLOT1

Experiment
siRNA / miRNA gene silencing Human - A2780 FLOT1
Product
Flotillin-1 siRNA (h) from Santa Cruz Biotechnology
Manufacturer
Santa Cruz Biotechnology

Protocol tips

Protocol tips
Incubate for 5 minutes at room temperature.

Incubate cells for 48h at 37°C
Downstream tips
Screen using western blot assay

Publication protocol

Notch-1 or FLOT1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (Shanghai, China). A2780 cells were transfected with Notch-1 or FLOT1 siRNAs and corresponding control siRNA for 48 h using Lipofectamine 2000 according to the manufacturer’s instructions. At 24 h after Notch-1 or control siRNA transfection, cells were split into 96-well plates and subjected to G418 (1 mg/ ml) selection for 2 weeks. The transcriptional silencing Notch-1 protein was screened using western blot assay. All transfection experiments were performed at least three times.

Full paper   Login or join for free to view the full paper.

Reviews

Flotillin-1 siRNA (h) from Santa Cruz Biotechnology has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - A2780 FLOT1 using Flotillin-1 siRNA (h) from Santa Cruz Biotechnology.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for Flotillin-1 siRNA (h) below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Human - A2780 FLOT1 using Flotillin-1 siRNA (h) from Santa Cruz Biotechnology. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms