Flotillin-1 siRNA (h)

siRNA / miRNA gene silencing Human - A2780 FLOT1

Experiment
siRNA / miRNA gene silencing Human - A2780 FLOT1
Product
Flotillin-1 siRNA (h) from Santa Cruz Biotechnology
Manufacturer
Santa Cruz Biotechnology

Protocol tips

Protocol tips
Incubate for 5 minutes at room temperature.

Incubate cells for 48h at 37°C
Downstream tips
Screen using western blot assay

Publication protocol

Notch-1 or FLOT1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (Shanghai, China). A2780 cells were transfected with Notch-1 or FLOT1 siRNAs and corresponding control siRNA for 48 h using Lipofectamine 2000 according to the manufacturer’s instructions. At 24 h after Notch-1 or control siRNA transfection, cells were split into 96-well plates and subjected to G418 (1 mg/ ml) selection for 2 weeks. The transcriptional silencing Notch-1 protein was screened using western blot assay. All transfection experiments were performed at least three times.

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Papers

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Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for Flotillin-1 siRNA (h) below.

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