Bim siRNA (h)

siRNA / miRNA gene silencing Human - A2780 Bim

Experiment
siRNA / miRNA gene silencing Human - A2780 Bim
Product
Bim siRNA (h) from Santa Cruz Biotechnology
Manufacturer
Santa Cruz Biotechnology

Protocol tips

Protocol tips
Add diluted DNA to diluted Lipofectamine® 2000 Reagent and incubate for 5 minutes at room temperature.

Incubate cells for 1–3 days at 37°C.
Downstream tips
Screen cells for western blot analysis and histone/DNA ELISA

Publication protocol

Akt and FOXO3a were purchased from Millipore; Bim and control small interfering RNA (siRNA) were purchased from Santa Cruz Biotechnology Inc. Human ovarian cancer A2780 and A2780/DDP cells were transfected with Akt, FOXO3a, Bim and control siRNA using Lipofectamine™ 2000, according to the manufacturer's instructions (31). The cells were then collected and processed for western blot analysis and histone/DNA ELISA.

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Papers

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Paper title
8-bromo-7-methoxychrysin induces apoptosis by regulating Akt/FOXO3a pathway in cisplatin-sensitive and resistant ovarian cancer cells
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Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for Bim siRNA (h) below.

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