SignalSilence® PTEN siRNA

siRNA / miRNA gene silencing Human - A2780 PTEN

Experiment
siRNA / miRNA gene silencing Human - A2780 PTEN
Product
SignalSilence® PTEN siRNA from Cell Signaling Technology
Manufacturer
Cell Signaling Technology

Protocol tips

Protocol tips
Add diluted DNA to diluted Lipofectamine® 2000 Reagent and incubate for 5 minutes at room temperature.

Incubate cells for 48h at 37°C.
Downstream tips
Screen cells for western blot analysis.

Publication protocol

A SignalSilence PTEN siRNA kit was purchased from Cell Signaling Technology (CST). A2780 cells were transfected with PTEN siRNA and negative control siRNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions. The transfection efficiency was evaluated by fluorescence microscopy by calculating the percentage of fluorescein-labelled cells. At 48 h after the transfection of the siPTEN, the cells were then subjected to viability and western blot assays for PTEN, pAkt.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - A2780 PTEN using SignalSilence® PTEN siRNA from Cell Signaling Technology.

Paper title
Berberine Sensitizes Human Ovarian Cancer Cells to Cisplatin Through miR-93/PTEN/Akt Signaling Pathway.
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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for SignalSilence® PTEN siRNA below.

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