ON-TARGETplus Human MET (4233) siRNA - SMARTpool

siRNA / miRNA gene silencing Human - A431 MET

Experiment
siRNA / miRNA gene silencing Human - A431 MET
Product
ON-TARGETplus Human MET (4233) siRNA - SMARTpool from Dharmacon
Manufacturer
Dharmacon

Protocol tips

Protocol tips
siRNA concentration: 30 nM–300 nM.

Post nucleofection,Incubate the cells in humidified 37°C/5% CO2 incubator until analysis.

Knockdown can be detected after 6–8 hours.

Publication protocol

Knockdown in H1299 and MDA MB231 was achieved by transfection of siRNA using Hiperfect (Qiagen) according to the protocol of the manufacturer, and constructs were expressed using Genejuice (Merck-Millipore) according to the protocol of the manufacturer. Knockdown and overexpression in HT29 and A431 cells were achieved by transfection of siRNA or plasmids using the AMAXA nucleofection method, solution V, and protocols X-001 and X-005, respectively.

The following siRNA oligos were used: control siRNA 1, 5′-GCAACGGCAUUCCACCUUU(TT)-3′; control siRNA 2 control pool of four siRNAs (Dharmacon, catalog no. D-001810-10−20); RCP (SMARTpool of four siRNAs (Dharmacon, catalog no. L-015968-00-0005); p53, 5′-GACUCCAGUGGUAAUCUAC(TT)-3′; p63 1, 5′-UGAACAGCAUGAACAAGCU(TT)-3′; p63 2, 5′-UGACUUCAACUUUGACAUG(TT)-3′; and MET (SMARTpool of four siRNAs (Dharmacon, catalog no. L-003156-00-0005); Dicer 1, 5′-GCU CGA AAU CUU ACG CAA A (TT)-3′; and Dicer 2, 5′-CCA CAC AUC UUC AAG ACU U (TT)-3′. All siRNA oligos were used as a combination of two or four siRNAs, and each individual siRNA was tested for efficiency of knockdown and off-target effects.

For siRNA rescue experiments, MDA MB231 and HT29 cells were transfected with the AMAXA nucleofection method, solution V, and protocols X-001 and X-005 using a combination of 2 μg of plasmid and 120 pmol of p53 siRNA for 8 × 106 cells.

Full paper   Login or join for free to view the full paper.

Reviews

ON-TARGETplus Human MET (4233) siRNA - SMARTpool from Dharmacon has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - A431 MET using ON-TARGETplus Human MET (4233) siRNA - SMARTpool from Dharmacon.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Dharmacon for ON-TARGETplus Human MET (4233) siRNA - SMARTpool below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Human - A431 MET using ON-TARGETplus Human MET (4233) siRNA - SMARTpool from Dharmacon. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms