PE Annexin V Apoptosis Detection Kit with 7-AAD

Apoptosis assay cell type - T-cells Mouse (OT-I)

Experiment
Apoptosis assay cell type - T-cells Mouse (OT-I)
Product
PE Annexin V Apoptosis Detection Kit with 7-AAD from BD Biosciences
Manufacturer
BD Biosciences

Protocol tips

Upstream tips
- Cells werepretreated with OVA-257 peptide SIINFEKL for the indicated time.
Protocol tips
- cells were co-activated by anti-CD3 and anti-CD28 Abs for the indicated time

Publication protocol

Cell proliferation and apoptosis rates were determined by flow cytometric analysis. For the analysis of proliferation, miR-491-overexpressing T cells and mock cells were isolated by density gradient centrifugation and labelled with carboxyfluorescein diacetate succinimidyl diester (CFSE) (5 μM, eBioscience) for 10 minutes at room temperature, and the reaction was stopped with one volume of foetal calf serum. Then, the cells were washed three times with PBS. These cells were continually cultured for another 48 hours under standard conditions, and the proliferation rate was assessed. For the apoptosis experiment, miR-491-overexpressing T cells and mock cells were co-activated by anti-CD3 and anti-CD28 Abs for the indicated time. OT-I T cells after transfection were isolated and restimulated with the OVA-257 peptide SIINFEKL for the indicated time. Then, cells were double-stained by annexin V/7AAD (BioLegend), and the rate of apoptosis was assessed. The results were analysed by FlowJo software version 6.2.

Full paper   Login or join for free to view the full paper.

Reviews

PE Annexin V Apoptosis Detection Kit with 7-AAD from BD Biosciences has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Apoptosis assay cell type - T-cells Mouse (OT-I) using PE Annexin V Apoptosis Detection Kit with 7-AAD from BD Biosciences.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from BD Biosciences for PE Annexin V Apoptosis Detection Kit with 7-AAD below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Apoptosis assay cell type - T-cells Mouse (OT-I) using PE Annexin V Apoptosis Detection Kit with 7-AAD from BD Biosciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms