ON-TARGETplus Human BSG / emmprin (682) siRNA - SMARTpool

siRNA / miRNA gene silencing Human - BCP-1 Emmprin / BSG

Experiment
siRNA / miRNA gene silencing Human - BCP-1 Emmprin / BSG
Product
ON-TARGETplus Human BSG / emmprin (682) siRNA - SMARTpool from Dharmacon
Manufacturer
Dharmacon

Protocol tips

Protocol tips
Mix the contents (dilute the siRNA and diluted DharmaFECT transfection reagent in serum-free medium) and incubate for 5 minutes at room temperature.

Add mixture to cells and incubate for 20 minutes at room temperature.

Replace culture medium with transfection medium and incubate cells at 37°C in 5% CO2 for 48 hours.

Publication protocol

Emmprin, LYVE-1, BCRP and non-target small interfering RNAs were purchased from the manufacturer (ON-TARGET plus SMART pool, Dharmacon, Lafayette, CO, USA). Cells were incubated with small interfering RNAs in 12-well plates using DharmaFECT Transfection Reagent (Dharmacon) according to the manufacturer’s instructions, and gene silencing assessed using immunoblots within 48 h.

Full paper   Login or join for free to view the full paper.

Reviews

ON-TARGETplus Human BSG / emmprin (682) siRNA - SMARTpool from Dharmacon has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - BCP-1 Emmprin / BSG using ON-TARGETplus Human BSG / emmprin (682) siRNA - SMARTpool from Dharmacon.

Paper title
Cooperative roles for emmprin and LYVE-1 in the regulation of chemoresistance for primary effusion lymphoma
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Dharmacon for ON-TARGETplus Human BSG / emmprin (682) siRNA - SMARTpool below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Human - BCP-1 Emmprin / BSG using ON-TARGETplus Human BSG / emmprin (682) siRNA - SMARTpool from Dharmacon. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms