siRNA p62 / SQSTM1

siRNA / miRNA gene silencing Human - BEAS-2B P62

Experiment
siRNA / miRNA gene silencing Human - BEAS-2B P62
Product
siRNA p62 / SQSTM1 from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
p62 siRNA; 5′ GGAGCACGGAGGGAAAAGAtt-3′;

siRNA concentration-50 nm

Add diluted siRNA to diluted
Lipofectamine® RNAiMAX
Reagent (1:1 ratio).

Incubate cells for 24 h at 37°C.

Publication protocol

Silencer predesigned small interference RNA (siRNA) for human p62 (siRNA ID s16960), Nrf2 (siRNA ID s9491), and control siRNA (AM4611) were obtained from Ambion (Austin, TX) and used to inhibit p62 and Nrf2 protein. The coding strand of p62 siRNA was 5′-GGAGCACGGAGGGAAAAGAtt-3′; the coding strand of Nrf2 siRNA was 5′-GAAUGGUCCUAAAACACCAtt-3′. Normal BEAS-2B cells and CdT cells were seeded in 96- or 6-well culture plates and transfected with 50 nm siRNA duplexes using LipofectamineTM RNAi MAX (Invitrogen) according to the manufacturer's instructions. Twenty-four hours after transfection, the cells were harvested, and cellular levels of proteins specific for the siRNA transfection were checked by immunoblotting.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - BEAS-2B P62 using siRNA p62 / SQSTM1 from Thermo Fisher Scientific.

Paper title
Nrf2/p62 Signaling in Apoptosis Resistance and Its Role in Cadmium-induced Carcinogenesis
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for siRNA p62 / SQSTM1 below.

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