siRNA FOXM1

siRNA / miRNA gene silencing Human - BT-20 FOXM1

Experiment
siRNA / miRNA gene silencing Human - BT-20 FOXM1
Product
siRNA FOXM1 from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Upstream tips
Seed 1.5–6 x 10^5 cells/weel in a 6 well plate.
Protocol tips
siRNA final concentration - 50 nM

Incuabte cells for 24h and add diluted siRNA to HiPerFect Transfection Reagent to make a mixture

Incubate the mixture for 5–10 min at room temperature.

Add mixture to cells and incubate for 72 h

Publication protocol

Two different FOXM1 small interfering RNAs (siRNAs) and non-silencing control siRNAs were purchased from Sigma-Aldrich. Exponentially growing untreated cells were plated 24 h before transfection. Plated cells were transfected with FOXM1 siRNA or a control siRNA at a final concentration of 50 nM for 72 h, using HiPerFect Transfection Reagent (Qiagen, Valencia, CA) according to the manufacturer's protocol. The concentrations of siRNAs were chosen based on dose-response studies. Non-silencing control siRNA-transfected cells were used as negative controls. After treatment, the cells were harvested and processed for further analysis.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - BT-20 FOXM1 using siRNA FOXM1 from Sigma-Aldrich.

Paper title
FOXM1 regulates expression of eukaryotic elongation factor 2 kinase and promotes proliferation, invasion and tumorgenesis of human triple negative breast cancer cells
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for siRNA FOXM1 below.

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