siRNA Junction plakoglobin (SASI_Hs01_00246520)

siRNA / miRNA gene silencing Human - BxPC-3 plakoglobin

Experiment
siRNA / miRNA gene silencing Human - BxPC-3 plakoglobin
Product
siRNA Junction plakoglobin (SASI_Hs01_00246520) from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Upstream tips
Seed 200 000 cells in 6 well plate for 24 hours
Protocol tips
siRNA concentration-40nm

Add diluted siRNA to diluted Lipofectamine Reagent

Incubate for 5 min at RT and add to cells.

Incubate cells for 1 day at 37°C and replace medium.

Incubate for another 48 hours.

Publication protocol

The day before transfection 200 000 cells were plated out in 6 well plates. Transfection were done with 40 nM of either MISSION siRNA Universal Negative Control #1 with no homology to all mature and predicted RefSeq mRNA sequences (SIC001, Sigma-Aldrich) or MISSION plakoglobin siRNA (ID: SASI_Hs01_00246520, Sigma-Aldrich) using 5 µl of LF2000 in a total of 2.5 ml growth medium without antibiotics. After 24 hours the medium was replaced and the cells were incubated for another 48 hours before analysis/harvesting.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - BxPC-3 plakoglobin using siRNA Junction plakoglobin (SASI_Hs01_00246520) from Sigma-Aldrich.

Paper title
Implications of Targeted Genomic Disruption of β-Catenin in BxPC-3 Pancreatic Adenocarcinoma Cells
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for siRNA Junction plakoglobin (SASI_Hs01_00246520) below.

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