|Seed 4–7 × 10^5 cells in t-25 flask|
|Final siRNA concentration - 50 nM.
After 72 h add diluted siRNA to diluted Lipofectamine Reagent
Incubate for 5 min at RT and add to cells.
Incubate cells for 4.5h at 37°C and replace medium with full growth medium and incubate for another 24h.
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