ON-TARGETplus Human DPAGT1 (1798) siRNA - SMARTpool

siRNA / miRNA gene silencing Human - CAL-27 DPAGT1

Experiment
siRNA / miRNA gene silencing Human - CAL-27 DPAGT1
Product
ON-TARGETplus Human DPAGT1 (1798) siRNA - SMARTpool from Dharmacon
Manufacturer
Dharmacon

Protocol tips

Protocol tips
siRNA concentration-150 nm.

Add diluted siRNA to diluted Lipofectamine Reagent

Incubate for 5 min at RT and add to cells.

Incubate cells for 2 days at 37°C.

Publication protocol

SMARTpool siRNAs targeting CTHRC1 and DPAGT1 (referred to as S siRNA) were obtained from Dharmacon. The non-silencing negative control siRNA (referred to as NS siRNA) was from Qiagen. CAL27 cells were transfected at 60% confluence with either NS or S siRNA (150 nm) using Lipofectamine 2000 (Invitrogen) and cultured for 48 h. Total RNAs isolated from CAL27 cells transfected with either NS or S siRNA were used for cDNA synthesis to assess CTHRC1 and DPAGT1 expression by real-time PCR. The gene expression profiles were generated by normalizing the Ct (threshold cycle numbers) of CTHRC1 and DPAGT1 with a housekeeping gene (18 S rRNA) and comparing the gene expression of cells treated with NS or S siRNA.

Full paper   Login or join for free to view the full paper.

Reviews

ON-TARGETplus Human DPAGT1 (1798) siRNA - SMARTpool from Dharmacon has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - CAL-27 DPAGT1 using ON-TARGETplus Human DPAGT1 (1798) siRNA - SMARTpool from Dharmacon.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Dharmacon for ON-TARGETplus Human DPAGT1 (1798) siRNA - SMARTpool below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Human - CAL-27 DPAGT1 using ON-TARGETplus Human DPAGT1 (1798) siRNA - SMARTpool from Dharmacon. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms