siRNA FOXL2

siRNA / miRNA gene silencing Human - COV-434 FOXL2

Experiment
siRNA / miRNA gene silencing Human - COV-434 FOXL2
Product
siRNA FOXL2 from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
Seed 300,000 cells per well of a six well plate.
Protocol tips
Add diluted siRNA to diluted Lipofectamine Reagent

Incubate for 5 min at RT and add to cells.

Incubate cells for 7h at 37°C and replace medium.

Publication protocol

For siRNA knockdown of mutant FOXL2, KGN cells, passage 8, were seeded at a density of 300,000 cells per well of a six well plate. Cells were transfected with FOXL2 stealth RNAi™ (cat#HSS101080 Invitrogen, NZ) or a GC matched control using Lipofectamine 2000 and Opti-MEM serum free media (Invitrogen, NZ). RNAi-lipid complexes were removed 7 h post transfection and replaced with complete media. For overexpression of wildtype or mutant FOXL2, COV434 cells, passage 9, were seeded at a density of 300,000 cells per well of a six well plate. Cells were transfected with 1 µg of control (empty vector), pFOXL2wt, or pFOXL2m using Lipofectamine 2000 and Opti-MEM serum free media. Plasmid-lipid complexes were removed 7 h post transfection and replaced with complete media. All transfections were performed in triplicate.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - COV-434 FOXL2 using siRNA FOXL2 from Thermo Fisher Scientific.

Paper title
The Transcriptional Targets of Mutant FOXL2 in Granulosa Cell Tumours
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for siRNA FOXL2 below.

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