Hs_GLI1_1 FlexiTube siRNA

siRNA / miRNA gene silencing Human - DAOY GLI1

Experiment
siRNA / miRNA gene silencing Human - DAOY GLI1
Product
Hs_GLI1_1 FlexiTube siRNA from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
Add diluted siRNA to diluted Lipofectamine® LTX Reagent (1:1 ratio).

Incubate for 5 minutes at room temperature and add to cells.

Incubate cells for 1–3 days at 37°C

Publication protocol

Predesigned GLI1 siRNA (Villegas et al., 2014) and nontargeting control siRNA (SIC001) were purchased from Sigma‐Aldrich (St. Louis, MO, USA), while siRNA targeting FOXS1 (sc‐75023) were purchased from Santa Cruz Biotechnologies (Dallas, TX, USA). Cells were plated in 6‐ or 24‐well plates at 50–70% confluency, and transfections were performed with Lipofectamine RNAimax (Invitrogen, Carlsbad, CA, USA) following the protocol provided by the manufacturer. Rh36 cells were transfected with expression constructs for GLI1 (Shimokawa et al., 2013), GLI1‐701G (Shimokawa et al., 2013), and 8 control pCMV‐based constructs or siRNA that did not cause significant change in GLI1 mRNA level. Transfections were performed using Lipofectamine LTX (Invitrogen) following manufacturer's instructions.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - DAOY GLI1 using Hs_GLI1_1 FlexiTube siRNA from Qiagen.

Paper title
Identification of novel GLI1 target genes and regulatory circuits in human cancer cells
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Manufacturer protocol

Download the product protocol from Qiagen for Hs_GLI1_1 FlexiTube siRNA below.

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