siRNA FOXS1

siRNA / miRNA gene silencing Human - DAOY FOXS1

Experiment
siRNA / miRNA gene silencing Human - DAOY FOXS1
Product
siRNA FOXS1 from Santa Cruz Biotechnology
Manufacturer
Santa Cruz Biotechnology

Protocol tips

Protocol tips
Add diluted siRNA to diluted Lipofectamine® LTX Reagent (1:1 ratio).

Incubate for 5 minutes at room temperature and add to cells.

Incubate cells for 1–3 days at 37°C

Publication protocol

Predesigned GLI1 siRNA (Villegas et al., 2014) and nontargeting control siRNA (SIC001) were purchased from Sigma‐Aldrich (St. Louis, MO, USA), while siRNA targeting FOXS1 (sc‐75023) were purchased from Santa Cruz Biotechnologies (Dallas, TX, USA). Cells were plated in 6‐ or 24‐well plates at 50–70% confluency, and transfections were performed with Lipofectamine RNAimax (Invitrogen, Carlsbad, CA, USA) following the protocol provided by the manufacturer. Rh36 cells were transfected with expression constructs for GLI1 (Shimokawa et al., 2013), GLI1‐701G (Shimokawa et al., 2013), and 8 control pCMV‐based constructs or siRNA that did not cause significant change in GLI1 mRNA level. Transfections were performed using Lipofectamine LTX (Invitrogen) following manufacturer's instructions.

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Papers

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Manufacturer protocol

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