siGENOME Human PPARD (5467) siRNA - SMARTpool

siRNA / miRNA gene silencing Human - DU145 PPAR-delta

Experiment
siRNA / miRNA gene silencing Human - DU145 PPAR-delta
Product
siGENOME Human PPARD (5467) siRNA - SMARTpool from Dharmacon
Manufacturer
Dharmacon

Protocol tips

Protocol tips
PPAR-delta siRNA concentration-40 pmol.

Incubate TransIT-TKO Reagent:siRNA complexe mixture at room temperature for 15–30 minutes to allow sufficient time for complexes to form and add to cells.

Incubate for 24–72 hours.

It is not necessary to replace the complete growth medium with fresh medium

Publication protocol

This method was previously reported [13]. In brief, duplexed RNA oligonucleotides for human PPAR-delta (siGENOME SMARTpool TM) were obtained from Thermo Fisher Scientific Inc., Taipei, Taiwan. DU145 cells were transfected with 40 pmol of PPAR-delta siRNA or scramble siRNA (scramble) using transfection reagents (TransIT-TKO, Mirus) as directed in the manufacturer’s protocols. The adequacy of PPAR-delta silencing was tested by immunoblot to optimize the experimental conditions (siRNA dose and time after transfection) according to previous reports [38–40].

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - DU145 PPAR-delta using siGENOME Human PPARD (5467) siRNA - SMARTpool from Dharmacon.

Paper title
Induction of apoptosis in prostate cancer by ginsenoside Rh2
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Manufacturer protocol

Download the product protocol from Dharmacon for siGENOME Human PPARD (5467) siRNA - SMARTpool below.

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