ON-TARGETplus Human MID1 (4281) siRNA - Individual

siRNA / miRNA gene silencing Human - DuCaP MID1

Experiment
siRNA / miRNA gene silencing Human - DuCaP MID1
Product
ON-TARGETplus Human MID1 (4281) siRNA - Individual from Dharmacon
Manufacturer
Dharmacon

Protocol tips

Protocol tips
MID1-3 siRNA: 5′-GUGUGAUACUAGGAUGCGG-3′

siRNA final concentration- 20 to 40 nM

Add diluted siRNA to diluted Lipofectamine Reagent

Incubate for 5 min at RT and add to cells.

Incubate cells for 1–3 days at 37°C.

Publication protocol

Cells were transfected with 20 to 40 nM final concentration of MID1, α4, AR or control siRNA oligonucleotides using Lipofectamin 2000 or Nanofectin. AR siRNA: 5′-GAAAGCUCCUCGGUAGGUC-3′; α4-4 siRNA: 5′-UUGAGAUGCCAUAGCAACGAG-3′; MID1-3 siRNA: 5′-GUGUGAUACUAGGAUGCGG-3′; ns siRNA: 5′-AAGAGGCUUGCACAGUGCA-3′ (Dharmacon or MWG). After 48 h of incubation the medium was replaced and after 72–120 h cells were harvested and analyzed by Western blotting or real-time PCR

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Papers

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Paper title
A hormone-dependent feedback-loop controls androgen receptor levels by limiting MID1, a novel translation enhancer and promoter of oncogenic signaling
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Manufacturer protocol

Download the product protocol from Dharmacon for ON-TARGETplus Human MID1 (4281) siRNA - Individual below.

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