|Twenty-four hours after transfection, cells were seeded in 96-well plates at either 3000 cells/well (QGY-7703 cells) or 5000 cells/well (SMMC-7721 cells). The WST-1 assay (Roche, Indianapolis, IN) was used to measure cell viability at 48 and 72 h after transfection. The absorbance at 450 nm was measured using a μQuant Universal Microplate Spectrophotometer (BioTek, Winooski, VT).|
Cell Proliferation Reagent WST-1 from Sigma-Aldrich has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
Check out relevant papers found by Labettor's AI that are relevant for performing Cell cytotoxicity / Proliferation assay cell type - SMMC-7721, Huh7, Hep3B, 293T using Cell Proliferation Reagent WST-1 from Sigma-Aldrich.
Download the product protocol from Sigma-Aldrich for Cell Proliferation Reagent WST-1 below.Download manufacturer protocol
Check out videos that might be relevant for performing Cell cytotoxicity / Proliferation assay cell type - SMMC-7721, Huh7, Hep3B, 293T using Cell Proliferation Reagent WST-1 from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.