Cell Proliferation Reagent WST-1

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	Twenty-four hours after transfection, cells were seeded in 96-well plates at either 3000 cells/well (QGY-7703 cells) or 5000 cells/well (SMMC-7721 cells). The WST-1 assay (Roche, Indianapolis, IN) was used to measure cell viability at 48 and 72 h after transfection. The absorbance at 450 nm was measured using a μQuant Universal Microplate Spectrophotometer (BioTek, Winooski, VT).

Protocol tips
Twenty-four hours after transfection, cells were seeded in 96-well plates at either 3000 cells/well (QGY-7703 cells) or 5000 cells/well (SMMC-7721 cells). The WST-1 assay (Roche, Indianapolis, IN) was used to measure cell viability at 48 and 72 h after transfection. The absorbance at 450 nm was measured using a μQuant Universal Microplate Spectrophotometer (BioTek, Winooski, VT).

Publication protocol

Twenty-four hours after transfection, cells were seeded in 96-well plates at either 3000 cells/well (QGY-7703 cells) or 5000 cells/well (SMMC-7721 cells). The WST-1 assay (Roche, Indianapolis, IN) was used to measure cell viability at 48 and 72 h after transfection. The absorbance at 450 nm was measured using a μQuant Universal Microplate Spectrophotometer (BioTek, Winooski, VT).

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