ON-TARGETplus Human MID1 (4281) siRNA - Individual

siRNA / miRNA gene silencing Human - LNCap MID1

Experiment
siRNA / miRNA gene silencing Human - LNCap MID1
Product
ON-TARGETplus Human MID1 (4281) siRNA - Individual from Dharmacon
Manufacturer
Dharmacon

Protocol tips

Protocol tips
Final concentration of MID1-20 to 40 nM.

MID1-3 siRNA: 5′-GUGUGAUACUAGGAUGCGG-3′

Add diluted siRNA to diluted NanoFectin reagent.

Incubate for 15 min at RT and add to cells. 

Replace medium after 48hand re-incubate cells for 72-120h at 37°C. 

Publication protocol

Cells were transfected with 20 to 40 nM final concentration of MID1, α4, AR or control siRNA oligonucleotides using Lipofectamin 2000 or Nanofectin. AR siRNA: 5′-GAAAGCUCCUCGGUAGGUC-3′; α4-4 siRNA: 5′-UUGAGAUGCCAUAGCAACGAG-3′; MID1-3 siRNA: 5′-GUGUGAUACUAGGAUGCGG-3′; ns siRNA: 5′-AAGAGGCUUGCACAGUGCA-3′ (Dharmacon or MWG). After 48 h of incubation the medium was replaced and after 72–120 h cells were harvested and analyzed by Western blotting or real-time PCR.

Full paper   Login or join for free to view the full paper.

Reviews

ON-TARGETplus Human MID1 (4281) siRNA - Individual from Dharmacon has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - LNCap MID1 using ON-TARGETplus Human MID1 (4281) siRNA - Individual from Dharmacon.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Dharmacon for ON-TARGETplus Human MID1 (4281) siRNA - Individual below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Human - LNCap MID1 using ON-TARGETplus Human MID1 (4281) siRNA - Individual from Dharmacon. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms