siRNA SKIL

siRNA / miRNA gene silencing Human - MCF-7 SKIL

Experiment
siRNA / miRNA gene silencing Human - MCF-7 SKIL
Product
siRNA SKIL from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
1:GGCAAGUAAGUCCAUAUCATT (sense) and UGAUAUGGACUUGCCTC (antisense)

2: GGCUCACAGUAGUGGUAATT (sense) and UUACCACUACUGUGAGCCTT (antisense)

siRNA concentartion-50 nM

Incubate transfection reagent:siRNA mixture for 10 min and add to cells.

Incubate the plate at 37°C for 24-72 h

Publication protocol

The knockdown of SKIL expression was done using small interfering RNA (AM16708, ID 107695) from Ambion (Ambion, Austin, TX, USA). The siRNA sequences (5′-to-3′) were:

1: GGCAAGUAAGUCCAUAUCATT (sense) and UGAUAUGGACUUGCCTC (antisense)

2: GGCUCACAGUAGUGGUAATT (sense) and UUACCACUACUGUGAGCCTT (antisense)

Silencer® Negative Control #1 siRNA (AM4611, Ambion, Austin, TX, USA) was used as a non-targeting control. Cells were seeded into 24-well plates (30000 cells/well) in four replicates and were transfected the following day with 50 nM SKIL siRNA and scrambled siRNA. INTERFERin TM (PolyPlus Transfection, Strasbourg, France) and Opti-MEM were used for cell transfection.

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Papers

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for siRNA SKIL below.

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