siRNA FOXM1

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Final concentration- 50 nM Add diluted siRNA to HiPerFect Transfection Reagent to make a mixture   Incubate the mixture for 5–10 min at room temperature.  Add mixture to cells and incubate for 72 h

Protocol tips
Final concentration- 50 nM Add diluted siRNA to HiPerFect Transfection Reagent to make a mixture   Incubate the mixture for 5–10 min at room temperature.  Add mixture to cells and incubate for 72 h

Publication protocol

Two different FOXM1 small interfering RNAs (siRNAs) and non-silencing control siRNAs were purchased from Sigma-Aldrich. Exponentially growing untreated cells were plated 24 h before transfection. Plated cells were transfected with FOXM1 siRNA or a control siRNA at a final concentration of 50 nM for 72 h, using HiPerFect Transfection Reagent (Qiagen, Valencia, CA) according to the manufacturer's protocol. The concentrations of siRNAs were chosen based on dose-response studies. Non-silencing control siRNA-transfected cells were used as negative controls. After treatment, the cells were harvested and processed for further analysis.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - MDA-MB-231 FOXM1 using siRNA FOXM1 from Sigma-Aldrich.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for siRNA FOXM1 below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Human - MDA-MB-231 FOXM1 using siRNA FOXM1 from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.