siRNA Wee1

siRNA / miRNA gene silencing Human - MDA-MB-231 Wee1

Experiment
siRNA / miRNA gene silencing Human - MDA-MB-231 Wee1
Product
siRNA Wee1 from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
siRNA concentration- 40 nM

Incubate the FuGENE 6 Transfection Reagent/medium mixture for 5 minutes at room temperature 

Incubate transfection mixture for 15 min at room temperature and add to cells and shake for 10-30 seconds. 

Incubate cells for 96 h. 

Publication protocol

MDA-MB-231 and SKOV3 cells were grown on glass coverslips in 6-well plates. If indicated, cells were treated with MK-1775 (500 nM) in the absence or presence of SU-9516 (1 µM). At 14 d after treatment, cells were fixed in 4% (wt/vol) paraformaldehyde in PBS. Subsequently, cells were stained with rat anti-CD44 (BioLegend 103002) combined with Alexa488-conjugated goat anti-rat secondary antibody and counterstained with DAPI. Images were obtained using a Leica DM6000B microscope equipped with a 10× objective (Leica HC PL FLUOTAR 10×/0.30) and LAS-AF software (Leica). In total 100–500 cells were scored per condition. Alternatively, cells were grown on glass coverslips and transfected with 40 nM Wee1 siRNA (#1 WEE1HSS111337 and #2 WEE1HSS111338; Invitrogen) or “medium GC duplex” control siRNA (Life Technologies 12935–300). At 96 h after siRNA transfection, cells were fixed and stained as described above.

Full paper   Login or join for free to view the full paper.

Reviews

siRNA Wee1 from Thermo Fisher Scientific has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - MDA-MB-231 Wee1 using siRNA Wee1 from Thermo Fisher Scientific.

Paper title
A haploid genetic screen identifies the G/S regulatory machinery as a determinant of Wee1 inhibitor sensitivity
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for siRNA Wee1 below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Human - MDA-MB-231 Wee1 using siRNA Wee1 from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms