ON-TARGETplus Human SLC7A11 (23657) siRNA - SMARTpool

siRNA / miRNA gene silencing Human - BCBL-1 xCT

Experiment
siRNA / miRNA gene silencing Human - BCBL-1 xCT
Product
ON-TARGETplus Human SLC7A11 (23657) siRNA - SMARTpool from Dharmacon
Manufacturer
Dharmacon

Protocol tips

Protocol tips
Mix the contents (dilute the siRNA and diluted DharmaFECT transfection reagent in serum-free medium) and incubate for 5 minutes at room temperature.

Add mixture to cells and incubate for 20 minutes at room temperature.

Replace culture medium with transfection medium and incubate cells at 37°C in 5% CO2 for 24 hours.

Publication protocol

For RNA interference assays, xCT ON-TARGET plus SMART pool siRNA (Dharmacon), or negative control siRNA, were delivered using the DharmaFECT transfection reagent according to the manufacturer’s instructions. To confirm initial transfection efficiency for siRNA experiments, PEL cells were transfected with green fluorescent protein (GFP)-tagged siRNA, and GFP expression determined by flow cytometry 24 h later as described previously [9]. Three independent transfections were performed for each experiment, and all samples were analyzed in triplicate for each transfection.

Full paper   Login or join for free to view the full paper.

Reviews

ON-TARGETplus Human SLC7A11 (23657) siRNA - SMARTpool from Dharmacon has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - BCBL-1 xCT using ON-TARGETplus Human SLC7A11 (23657) siRNA - SMARTpool from Dharmacon.

Paper title
Targeting xCT, a cystine-glutamate transporter induces apoptosis and tumor regression for KSHV/HIV-associated lymphoma
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Dharmacon for ON-TARGETplus Human SLC7A11 (23657) siRNA - SMARTpool below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Human - BCBL-1 xCT using ON-TARGETplus Human SLC7A11 (23657) siRNA - SMARTpool from Dharmacon. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms