|Seed 150,000 cells/well|
|siRNA concentration- 5–100 nmol/l
si-hVDAC1- S: 238-5′ACACUAGGCACCGAGAUUA3′-256 and AS: 5′UAAUCUCGGUGCCUAGUGU3′; si-hVDAC1 1/A- S: -238-5′ACACUAGGCACCGAGAUUA 3′- 256, and AS: 5′UAAUCUCGGUGCCUAGUGU3′-256; si-hVDAC1 2/A- S: 238-5′ ACACUAGGCACCGAGAUUA3′-256 and AS: 238-5′UAAUCUCGGUGCCUAGUGU3′; si-hVDAC1 2/B-, S: 238- 5′ACACUAGGCACCGAGA UUA3′-256 and AS: 238-5′UAAUCUCGGUGCCUAGUGU3′-256
Mix the contents (dilute the siRNA and diluted DharmaFECT transfection reagent in serum-free medium) and incubate for 5 minutes at room temperature.
Add mixture to cells and incubate for 20 minutes at room temperature.
Replace culture medium with transfection medium and incubate cells at 37°C in 5% CO2 for 48 hours.
Accell Human VDAC1 (7416) siRNA - Set of 4 from Dharmacon has not yet been reviewed for this experiment
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