DeadEnd™ Colorimetric TUNEL System

TUNEL assay cell type - HNSCC Detroit 562 human head and neck tumor cells

Experiment
TUNEL assay cell type - HNSCC Detroit 562 human head and neck tumor cells
Product
DeadEnd™ Colorimetric TUNEL System from Promega
Manufacturer
Promega

Protocol tips

Upstream tips
- Store the Equilibration Buffer, rTdT Enzyme, Biotinylated Nucleotide Mix and Proteinase K at –20°C
Downstream tips
- If high background, Increase blocking time with 0.3% hydrogen peroxide

Publication protocol

Formalin-fixed tumor samples were paraffin-embedded and used for IHC analyses of PCNA and phospho-H2A.X (Ser139) following the earlier described methods (25). The apoptotic cell population in the tumor sections was determined by DeadEnd Colorimetric TUNEL assay kit (Promega, Madison, WI). The percent positive stained cells were determined as number of positive cells (brown stained) × 100/total number of cells in five randomly selected area per tumor section at ×400 magnification, and images were processed by Zeiss AxioVision software documentation system (Carl Zeiss, Jena, Germany).

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Discussion

Discussion

3 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

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Manufacturer protocol

Download the product protocol from Promega for DeadEnd™ Colorimetric TUNEL System below.

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