siRNA RUNX3

siRNA / miRNA gene silencing Human - KLM-1 RUNX3

Experiment
siRNA / miRNA gene silencing Human - KLM-1 RUNX3
Product
siRNA RUNX3 from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
Vortex complex mixture (RNAiFect Transfection Reagent and diluted siRNA) for 10 seconds

Incubate the samples for 10–15 min at room temperature (15–25°C) to allow formation of transfection complexes and add to cells.

Incubate cells with serum starved for 48 h.

Publication protocol

RUNX3‐expressing SUIT‐2 and KLM‐1 cells were transfected with either scrambled negative control small interfering RNA (siRNA) or RUNX3 siRNA (Applied Biosystems, Foster City, CA) by using RNAiFect™ transfection reagent (Qiagen, Hilden, Germany). The cells were incubated for 24 h and then serum starved for 48 h. The MTT assay was performed as described in Section 2.8.

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Papers

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for siRNA RUNX3 below.

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