Dansylcadaverine

Protocol tips

Upstream tips	Protocol tips	Downstream tips
MDC is an autofluorescent agent used as selective marker for autophagic vacuoles and especially for autolysosomes.	Stain the cells with 0.05 mmol/L MDC at 37oC for 1 h.

Upstream tips
MDC is an autofluorescent agent used as selective marker for autophagic vacuoles and especially for autolysosomes.
Protocol tips
Stain the cells with 0.05 mmol/L MDC at 37oC for 1 h.

Publication protocol

A fluorescent compound, monodansylcadaverine (MDC) has been proposed as a tracer for autophagic vacuoles. L929 cells were treated with 80 µmol/L PAB for 3 days, then were incubated with 0.05 mmol/L MDC at 37 ℃ for 1 h. After incubation, cells were washed once with PBS. Intracellular MDC was measured by fluorescence microscopy at excitation wave length 380 nm with emission filter 525 nm (Leica, Nusslich, Germany).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Autophagy assay cell type - L929 using Dansylcadaverine from Sigma-Aldrich.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Dansylcadaverine below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Autophagy assay cell type - L929 using Dansylcadaverine from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.