Dansylcadaverine

Autophagy assay cell type - HT1080

Experiment
Autophagy assay cell type - HT1080
Product
Dansylcadaverine from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Upstream tips
An autofluorescent dye that accumulates in the autophagic vacuoles.
Protocol tips
50 mM MDC for 40 min at 37uC, washed twice with PBS, and then observed by a fluorescent microscope.

Publication protocol

MDC, a fluorescent compound, was used as a tracer for autophagic vacuoles. After incubation with KIOM-C, cells were stained with 50 µM MDC for 40 min at 37°C, washed twice with PBS, and then observed by a fluorescent microscope

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Papers

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Paper title
A Novel Herbal Medicine, KIOM-C, Induces Autophagic and Apoptotic Cell Death Mediated by Activation of JNK and Reactive Oxygen Species in HT1080 Human Fibrosarcoma Cells
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Dansylcadaverine below.

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