LC3B (D11) XP® Rabbit mAb

Autophagy assay cell type - HT1080

Experiment
Autophagy assay cell type - HT1080
Product
LC3B (D11) XP® Rabbit mAb from Cell Signaling Technology
Manufacturer
Cell Signaling Technology

Protocol tips

Protocol tips
Dilute primary Ab at 1:200

Publication protocol

A Nikon Eclipse E800 Fluorescence microscope was used. Cells were plated on coverslips one night before being transfected with indicated constructs in Opti-MEM using FuGENE HD reagent. 24 h after treatment, slides were fixed using 4% paraformaldehyde/PBS at room temperature for 15 min and washed twice with PBS. For immunofluorescent analysis, slides were then washed twice with PBS, permeabilized by using PBS containing 0.25% Triton X-100, and blocked with 2% BSA in PBST for 30 min, and the first antibodies were added following Abcam immunofluorescence protocol. The autophagic process was analyzed by counting percentage of cells showing accumulation of GFP (RFP) LC3 puncta and the average number of GFP (RFP) LC3 puncta per cell. For the calculation of percentage of cells showing LC3 accumulation, a total of 300 cells were calculated each time for three duplicates. For the calculation of average number of puncta per cell, a total number of 100 cells were calculated each time for three duplicates. The representative fluorescent images were collected using SPOT Advanced software.

Full paper   Login or join for free to view the full paper.

Reviews

LC3B (D11) XP® Rabbit mAb from Cell Signaling Technology has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Autophagy assay cell type - HT1080 using LC3B (D11) XP® Rabbit mAb from Cell Signaling Technology.

Paper title
Tid1, the Mammalian Homologue of Tumor Suppressor Tid56, Mediates Macroautophagy by Interacting with Beclin1-containing Autophagy Protein Complex
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Cell Signaling Technology for LC3B (D11) XP® Rabbit mAb below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Autophagy assay cell type - HT1080 using LC3B (D11) XP® Rabbit mAb from Cell Signaling Technology. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms