Beclin 1 Rabbit Polyclonal antibody

Autophagy assay cell type - A375

Experiment
Autophagy assay cell type - A375
Product
Beclin 1 Rabbit Polyclonal antibody from Proteintech Group
Manufacturer
Proteintech Group

Protocol tips

Upstream tips
- Lyse cells using RIPA mixed with protease inhibitors and phosphatase inhibitors
Protocol tips
- Dilute Primary Ab at 1:500-1:3000

Publication protocol

A density of 1 × 105 A375 cells were seeded onto 6-well plates. After 24 h, cells were treated with varying concentrations of CP for 12 h, 24 h and 48 h. Then cells were washed twice immediately with pre-chilled PBS on ice. The cytoplasmic proteins were lysed using RIPA mixed with protease inhibitors and phosphatase inhibitors (Roche, Basel, Switzerland). The protein concentration was measured by the BCA protein assay kit (Fudebio, Hangzhou, China). The protocol we used was that same with our previous publication [38].

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Papers

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Manufacturer protocol

Download the product protocol from Proteintech Group for Beclin 1 Rabbit Polyclonal antibody below.

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