CYTO-ID® Autophagy detection kit

Autophagy assay cell type - Blood

Experiment
Autophagy assay cell type - Blood
Product
CYTO-ID® Autophagy detection kit from Enzo Life Sciences
Manufacturer
Enzo Life Sciences

Protocol tips

Downstream tips
- Incase of Low CYTO-ID Green dye staining in all treatments, increase the reagent concentration (500X dilution of the dye is recommended) and the incubation time.

- Incase of High CYTO-ID Green staining, it could be that the cell culture medium was depleted of nutrients and so change media 4-8 hours before the experiment.

Publication protocol

CYTO-ID Autophagy detection kit (Enzo Life Sciences, Lausen, Switzerland) is another commercially available tool to measure autophagy by FC. The probe used in this kit is a cationic amphiphilic tracer dye that primarily stains autophagosomes, while staining lysosomes minimally. This dye has been successfully used in both cell lines and primary cells from human and mouse origin (summarized in the company’s website). It can be used in conjunction with surface markers that discriminate different cell populations

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Reviews

CYTO-ID® Autophagy detection kit from Enzo Life Sciences has not yet been reviewed for this experiment

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Autophagy assay cell type - Blood using CYTO-ID® Autophagy detection kit from Enzo Life Sciences.

Paper title
Assessing Autophagy in Mouse Models and Patients with Systemic Autoimmune Diseases
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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for CYTO-ID® Autophagy detection kit below.

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Videos

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