|This kit uses a green fluorescent probe that reacts with autophagic vacuoles and is detectable by flow cytometry|
|Incubate for 30 minutes at 37°C in the dark|
|Incase of Low CYTO-ID Green dye staining in all treatments, increase the reagent concentration (500X dilution of the dye is recommended) and the incubation time.
Incase of High CYTO-ID Green staining, it could be that the cell culture medium was depleted of nutrients and so change media 4-8 hours before the experiment
CYTO-ID® Autophagy detection kit from Enzo Life Sciences has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
Check out relevant papers found by Labettor's AI that are relevant for performing Autophagy assay cell type - MCF10A using CYTO-ID® Autophagy detection kit from Enzo Life Sciences.
Download the product protocol from Enzo Life Sciences for CYTO-ID® Autophagy detection kit below.Download manufacturer protocol
Check out videos that might be relevant for performing Autophagy assay cell type - MCF10A using CYTO-ID® Autophagy detection kit from Enzo Life Sciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.