LC3 Antibody (APG8B) (N-term)

Autophagy assay cell type - KYSE450

Experiment
Autophagy assay cell type - KYSE450
Product
LC3 Antibody (APG8B) (N-term) from Abgent
Manufacturer
Abgent

Protocol tips

Upstream tips
This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LC3.

Publication protocol

Cells were fixed in 4% paraformaldehyde and incubated with anti-LC3 (Abgent, Oxfordshire, UK) or anti-caspase 3 (Cell Signaling, Danvers, MA, USA) and AlexaFluor-488 secondary antibody with standard washing procedures (Invitrogen, Paisley, UK). Analysis was carried out using an Olympus Fluorescence (Southend-on-Sea, Essex, UK) microscope.

Full paper   Login or join for free to view the full paper.

Reviews

LC3 Antibody (APG8B) (N-term) from Abgent has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Autophagy assay cell type - KYSE450 using LC3 Antibody (APG8B) (N-term) from Abgent.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Abgent for LC3 Antibody (APG8B) (N-term) below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Autophagy assay cell type - KYSE450 using LC3 Antibody (APG8B) (N-term) from Abgent. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms