DMEM–Dulbecco's Modified Eagle Medium

Mammalian cell culture media DH82

Experiment
Mammalian cell culture media DH82
Product
DMEM–Dulbecco's Modified Eagle Medium from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
Dulbecco's Modified Eagle Medium (Life Technologies; UK) containing 10% foetal bovine serum (FBS), 0.2% amphotericin B (2 µg/ml), and 1% penicillin (100 U/ml) streptomycin (100 µg/ml) at 37°C in 5% CO2.

Publication protocol

Forelimbs from three horses were collected from a local abattoir. The metacarpophalangeal joint was cleaned and skinned before opening. The joint surface was scored macroscopically to ensure absence of orthopaedic disease.3 Synovium was dissected, finely diced, and cells isolated using 0.25% (w/v) trypsin/0.1% (w/v) EDTA digestion followed by incubation with 2 mg/ml collagenase type 2 (Worthington Biochemicals; Berkshire, UK) Cells were seeded at 6 × 104 cells/cm2 and expanded for up to three passages using split ratios of 1:3. Unless stated otherwise, all cells were cultured in Dulbecco's Modified Eagle Medium (Life Technologies; UK) containing 10% foetal bovine serum (FBS), 0.2% amphotericin B (2 µg/ml), and 1% penicillin (100 U/ml) streptomycin (100 µg/ml) at 37°C in 5% CO2.

Full paper   Login or join for free to view the full paper.

Reviews

DMEM–Dulbecco's Modified Eagle Medium from Thermo Fisher Scientific has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Mammalian cell culture media DH82 using DMEM–Dulbecco's Modified Eagle Medium from Thermo Fisher Scientific.

Paper title
Interaction with macrophages attenuates equine fibroblast‐like synoviocyte ADAMTS5 (aggrecanase‐2) gene expression following inflammatory stimulation
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for DMEM–Dulbecco's Modified Eagle Medium below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Mammalian cell culture media DH82 using DMEM–Dulbecco's Modified Eagle Medium from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms