MEM (Eagle) Liquid Medium

Mammalian cell culture media MDCK

Experiment
Mammalian cell culture media MDCK
Product
MEM (Eagle) Liquid Medium from Merck Millipore
Manufacturer
Merck Millipore

Protocol tips

Protocol tips
MDCK II cells were maintained in Earle’s minimum essential medium supplemented with 4 mM glutamine, 0.2 mg/mL of both penicillin and streptomycin (Biochrom, Berlin, Germany), 10% (v/v) fetal calf serum (PAA, Pasching, Austria) in a 5% CO2 humidified incubator (HERA cell 150, Heraeus, Germany). Cells were subcultured weekly after reaching confluence by washing with PBS, followed by trypsinization and centrifugation at 110g.

Publication protocol

MDCK II cells were maintained in Earle’s minimum essential medium supplemented with 4 mM glutamine, 0.2 mg/mL of both penicillin and streptomycin (Biochrom, Berlin, Germany), 10% (v/v) fetal calf serum (PAA, Pasching, Austria) in a 5% CO2 humidified incubator (HERA cell 150, Heraeus, Germany). Cells were subcultured weekly after reaching confluence by washing with PBS, followed by trypsinization and centrifugation at 110g.

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Papers

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Paper title
Mechanical properties of MDCK II cells exposed to gold nanorods
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Manufacturer protocol

Download the product protocol from Merck Millipore for MEM (Eagle) Liquid Medium below.

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