RPMI 1640

Mammalian cell culture media CHO

Experiment
Mammalian cell culture media CHO
Product
RPMI 1640 from Nacalai Tesque
Manufacturer
Nacalai Tesque

Protocol tips

Protocol tips
supplemented with 10% heat-inactivated fetal bovine serum (Thermo Fisher Scientific Inc.), 100 units/mL of penicillin, 100 μg/mL of streptomycin, and 25 μg/mL of amphotericin B (Nacalai Tesque, Inc.)

Publication protocol

P3U1, CHO-K1, and CHO/horPDPN cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Nacalai Tesque, Inc., Kyoto, Japan), and FHK-Tcl3.1 was cultured in Dulbecco's modified Eagle's medium (DMEM; Nacalai Tesque, Inc.) [32]. All media were supplemented with 10% heat-inactivated fetal bovine serum (Thermo Fisher Scientific Inc.), 100 units/mL of penicillin, 100 μg/mL of streptomycin, and 25 μg/mL of amphotericin B (Nacalai Tesque, Inc.). Cells were grown at 37 °C in a humidified environment with an atmosphere of 5% CO2 and 95% ambient air.

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Papers

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Paper title
PMab-219: A monoclonal antibody for the immunohistochemical analysis of horse podoplanin
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Manufacturer protocol

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