RPMI-1640 Medium

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	supplemented with 10% fetal calf serum, 100 U/ml penicillin, 100 μg/ml streptomycin

Protocol tips
supplemented with 10% fetal calf serum, 100 U/ml penicillin, 100 μg/ml streptomycin

Publication protocol

JURL-MK1, CML-T1 and Karpas-299 cell lines were purchased from DSMZ (German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany), K562, HL-60 and Jurkat cell lines from the European Collection of Animal Cell Cultures (Salisbury, UK). MOLM-7 and HEL cells were provided by J.Minowada [21] and P.Martin [22], respectively. All cell lines were cultured in RPMI 1640 medium supplemented with 10% fetal calf serum, 100 U/ml penicillin, 100 μg/ml streptomycin at 37°C in 5% CO2 humidified atmosphere. The cells were counted using the automated cell counter TC10 (BioRad). As indicated by the counter, the main cell diameter was 12 μm for the majority of cell lines except for CML-T1 cells (8 μm) and K562 and HEL cells (both 16 μm).

Primary blood cells were obtained from the whole blood of healthy donors or from leukapheresis product from patients with chronic or acute myelogenous leukemia. Peripheral blood mononuclear cells (PBMC) were separated by standard density gradient centrifugation using Histopaque-1077 (Sigma) and maintained in RPMI 1640 medium described above.

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Manufacturer protocol

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